Posted by
Cameron Nowell-2 on
Jul 17, 2013; 10:12pm
URL: http://imagej.273.s1.nabble.com/Quantification-of-red-in-bright-field-image-tp5003997p5004016.html
I would only stick to area coverage. IOD, intensity etc on chromogenic stains can be varied. They are very rarely stoichiometric and changes in intensity can just be due to slightly longer incubation times.
Cheers
Cam
________________________________________
From: ImageJ Interest Group [
[hidden email]] on behalf of Karsten [
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Sent: Thursday, July 18, 2013 12:07 AM
To:
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Subject: Re: Quantification of red in bright field image
To measure the "amount" of red it might be good to estimate from the segmented/thresholded region the total extinction, that is the integrated density of the extinction of red image, Extinction = -log(Transmission red / 255). The so called IOD (integrated optical density) represents the amount of absorbing material!
Regards
Karsten
Am 17.07.2013 um 14:57 schrieb Cameron Nowell <
[hidden email]>:
> Hi Louise,
>
> It can be done easily enough. I use the Fiji distribution of ImageJ (www.fiji.sc) so i am not sure if the functionality is available in the standard imagej package but the following worked for me
>
> 1. go to Image --> colour --> colour deconvolution
> 2. choose H AEC as te vector and press OK
> 3. you should get three images. Select the red channel one (Colour_2)
> 4. go to image adjust threshold, Make sure dark background is unselected. Adjust the threshold (or the MaxEntropy auto setting seemed to work ok).
> 5. Measure the image (Analyze --> Measure)
>
>
> If you want a macro for doing this let me know
>
>
> Cheers
>
> Cam
>
>
> Cameron J. Nowell
> Research Facilities Manager
>
> Monash Institute of Pharmaceutical Sciences
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> ________________________________________
> From: ImageJ Interest Group [
[hidden email]] on behalf of Louise Grant [
[hidden email]]
> Sent: Wednesday, July 17, 2013 8:27 PM
> To:
[hidden email]
> Subject: Quantification of red in bright field image
>
> Hello,
>
>
>
> I`d like to quantify the amount of red in this histo slide using ImageJ. I
> am not experience with microscopes and I´m not sure where to start. I
> searched the ImageJ archives but everything seems to be for fluorescent
> images and not brightfield.
>
>
>
> Any help would be great!
>
>
>
> Thanks
>
>
>
> Louise
>
>
>
>
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http://imagej.nih.gov/ij/list.htmlKarsten
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