Re: measuring grayscale of objects in a plate

Posted by JDing on Jul 26, 2013; 8:55pm
URL: http://imagej.273.s1.nabble.com/measuring-grayscale-of-objects-in-a-plate-tp5004160p5004163.html

You understand me correctly, but your suggestion doesn't seem to work. I added "center of mass" to the measurements and it didn't put the wells in order. Here is my explanation: even though the sides of my plate are aligned with the x and y axes, the seeds themselves vary in their position within the well (and their size). So the seed in A1 could be centered at coordinate (50, 50), while B1 is at (100, 49), and C1 is at (150, 49.5), etc. In this case, if ImageJ measured/listed in order of increasing y-coordinate (the default for my computer), it would list B1 and C1 before A1. So this method doesn't work well in real practice.

Thank you for your consideration, though.

JDing