http://imagej.273.s1.nabble.com/measuring-grayscale-of-objects-in-a-plate-tp5004160p5004164.html
Assuming your wells build a chequerboard and the image is aligned with it, the coordinates of your ROIs will allow the ordering of the results. Of course the actual list of result entries depend on the sequence of your ROIs in the ROI manager rspw. on the first found pixel per region.
Possibly you have to load the results into Excel or R for the ordering in x and y.
> You understand me correctly, but your suggestion doesn't seem to work. I
> added "center of mass" to the measurements and it didn't put the wells in
> order. Here is my explanation: even though the sides of my plate are aligned
> with the x and y axes, the seeds themselves vary in their position within
> the well (and their size). So the seed in A1 could be centered at coordinate
> (50, 50), while B1 is at (100, 49), and C1 is at (150, 49.5), etc. In this
> case, if ImageJ measured/listed in order of increasing y-coordinate (the
> default for my computer), it would list B1 and C1 before A1. So this method
> doesn't work well in real practice.
>
> Thank you for your consideration, though.
>
> JDing
>
>
>
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