Re: measuring grayscale of objects in a plate

Posted by Gabriel Landini on Jul 27, 2013; 8:37am
URL: http://imagej.273.s1.nabble.com/measuring-grayscale-of-objects-in-a-plate-tp5004160p5004174.html

On Saturday 27 Jul 2013 01:21:06 JDing wrote:
> Maybe an illustrative example will clear up the confusion:

I think you are complicating things unnecessarily. The order of the analysis
is on the x,y scan order of the image, so unless you have things aligned in a
pixel-accurate manner you can't tell easily which is the next seed that will
be found.
Perhaps it would be easier to break down the image into a stack with NxM
slices with one object each and analyse one slice at a time. There is an
unmontage command that does the reverse of the montage command.
That way it does not matter where within the sub-image the seeds are.
Cheers

Gabriel

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