Re: measuring grayscale of objects in a plate

Posted by JDing on Jul 27, 2013; 5:33pm
URL: http://imagej.273.s1.nabble.com/measuring-grayscale-of-objects-in-a-plate-tp5004160p5004181.html

Gabriel, I think your idea would work, but I can't find any unmontage command in ImageJ. Perhaps it is known by a different name?

Your idea led me to try a related idea: I used my saved ROI set to crop each well one at a time, in the correct order, and then I apply threshold/analyze particles and measure the new ROI generated for the seed. This way the results table will have measurements in the correct order. I believe this should work (I just tried it for the first well, and it worked), and I'll run a full test on Monday. I'll keep you updated if I run into any trouble.

Thank you for your very helpful suggestions.

JDing

On Sat, Jul 27, 2013 at 3:54 AM, Gabriel Landini [via ImageJ] <[hidden email]> wrote:

On Saturday 27 Jul 2013 01:21:06 JDing wrote:
> Maybe an illustrative example will clear up the confusion:

I think you are complicating things unnecessarily. The order of the analysis
is on the x,y scan order of the image, so unless you have things aligned in a
pixel-accurate manner you can't tell easily which is the next seed that will
be found.
Perhaps it would be easier to break down the image into a stack with NxM
slices with one object each and analyse one slice at a time. There is an
unmontage command that does the reverse of the montage command.
That way it does not matter where within the sub-image the seeds are.
Cheers

Gabriel

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