Re: Isolating the background pixels from foreground pixels

Posted by Geology Guy on
URL: http://imagej.273.s1.nabble.com/Isolating-the-background-pixels-from-foreground-pixels-tp5003764p5004207.html

Dear Karsten,
I was having a look at the Shape descriptor values that I got for the images that I processed and found out that the value for Aspect Ratio was above 1. So in the particle summary Circ and Solidity were reported but not Aspect ratio and Roundness (Round). Is this because I did a bit of thresholding and water shed segmentation on the image or do you can you tell me the cause of these high values?

Thank you


On Sat, Jul 27, 2013 at 7:23 AM, Tola Ogunniyi <[hidden email]> wrote:
Dear Karsten,
It was more of my Professor's choice for me to collect all the data regardless of whether I need it or not, but I will focus on the ones you have suggested for now. I thank you very much for responding on time and I hope you have a great day.


On Sat, Jul 27, 2013 at 6:40 AM, Karsten Rodenacker-3 [via ImageJ] <[hidden email]> wrote:
Hi Geology Guy,

Seemingly you have switched on all features. For the shape of particles there are IMHO only senseful:

Area (*)
Perimeter (*)
Circ (*)
Solidity (*)
Round (*)
Feret (*)
MinFeret
Minor/Major

all others are related to redirection to gray scale image or not really helpful for particle shape description, better for representation e.g. by ellipsoids.

To summarize a set of particles:

The features (*) can be summarized by the usual Mean and SD.
 
Min Feret is special, either the (global) Minimum or the span (Max-Min) could characterize the set os particles.

Feret Angle and Angle might be helpful for elongated particles, but the mean of angles is a difficult task since angles come from a number module. (to difficult for the available time!)

The mean of (Minor/Major), a ratio estimator is mean(Minor)/mean(Major) not mean(Minor/Major). SD of the ratio is a bit complicated too!

I think this list of features should be enough for characterization of a set of particles!

Hope that helps a bit

Regards
Karsten

Am 27.07.2013 um 03:47 schrieb Geology Guy <[hidden email]>:

> Dear Karsten,
>
> I am trying to measure the parameters of 100 particles in Image J and under

> "Analyze Particles", I also selected the "Summary option". My main problem
> now is that I deleted some of the particles as requested by my Professor
> from the list after the data was provided by Image J. This makes the
> "Summary" data not useful anymore as they do not reflect all the 100
> particles I started with originally. I measured all the parameters under
> "Set measurement" for each particle so now I need to produce a new summary
> data as the values would be used in plotting graphs. I have figured out how
> to Area which is just a simple addition of the areas of each particle.
> Other parameters that are in the summary that I cannot determine from the
> particles data are the following:
>
>
>
>
>
>
>
> Perim.
>
> Major
>
> Minor
>
> Circ.
>
> Solidity
>
> Feret
>
> FeretX
>
> FeretY
>
> FeretAngle
>
> MinFeret
>
> IntDen
>
> Median
>
> Skew
>
> Kurt
>
>
>
> I have attached 2 Excel spreadsheets. The first shows my original particles
> data and the highlighted rows in yellow are rows that I have deleted their
> data as they are bad data. I am working on my research that is due on the
> 31st of this month so this is very urgent. Thank you very much for your
> assistance.
>
>
> Yours faithfully,
>
> Tola
>
>
> On Thu, Jul 4, 2013 at 5:10 PM, Karsten Rodenacker-3 [via ImageJ] <
> [hidden email]> wrote:

>
>> Hi Geology Guy, possibly geologists think different.
>>
>> There are gray or color images with bright background and others with a
>> dark background. This should not be mixed up with the representation of
>> binary images. Foreground is always the set of pixels which are thresholded
>> (shown with the small box) in the histogram of the interactive thresholding
>> window. They get a value of 255. The background gets 0. Still by the
>> Options->Colors… you can select the representation of foreground and
>> background pixels.
>>
>> Maybe some clarification for you?
>>
>> Karsten
>>
>> (I have to confess that I rarely read manuals, I prefer trial and error)
>>
>> Am 04.07.2013 um 22:49 schrieb Geology Guy <[hidden email]<http://user/SendEmail.jtp?type=node&node=5003767&i=0>>:
>>

>>
>>> Dear Karsten,
>>> Thanks for your response. I checked the Edit> Options> Colors and the
>>> background is black while the Foreground is White. This is what I expect
>>> because after reading the Image J manual, I learnt that Image J only
>> makes
>>> calculations from binary images when the background is black and the
>>> foreground is white.
>>>
>>> I am using the default thresholding (i.e. AUTO) and checked the DARK
>>> BACKGROUND BOX. Then when I was ready to do binary image a,dialogue box
>>> popped up and I unchecked the box next to "BLACK FOREGROUND and WHITE
>>> BACKGROUND". Again this setting that I am using is because the Image J
>>> manual recommended a background that is black and white foreground in
>> order
>>> to be able to carry out calculations involving binary imaging. Or am I
>> wrong
>>> somewhere maybe that's why the background pixels are getting picked up
>> in
>>> the foreground when I try to calculate Area for foreground objects ONLY?
>>> Thanks for your help.
>>>
>>>
>>>
>>>
>>>
>>> --
>>> View this message in context:
>> http://imagej.1557.x6.nabble.com/Isolating-the-background-pixels-from-foreground-pixels-tp5003764p5003766.html
>>> Sent from the ImageJ mailing list archive at Nabble.com.
>>>
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>>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
>> Karsten
>> [hidden email] <http://user/SendEmail.jtp?type=node&node=5003767&i=1>
>>
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