Re: Make Binary, Analyze Particles (different results on different systems)

Posted by Rainer M. Engel on Aug 05, 2013; 2:06pm
URL: http://imagej.273.s1.nabble.com/Make-Binary-Analyze-Particles-different-results-on-different-systems-tp5004298p5004303.html

Am 05.08.2013 12:17, schrieb Gabriel Landini:

> On Monday 05 Aug 2013 10:46:28 you wrote:
>> I got different results when converting a mask to binary. On one system
>> it gets inverted (inverting LUT) on the other it stays the same.
>>
>> On one system I can run "Analyze Particles" and get one element in
>> canvas middle, on the other the whole canvas is detected.
>>
>> I read about the "Black background" Option of the Binary menu.
>> Process > Binary > Options and remembered having heard of that before.
>> And since I wrote this mail to get help in the first place, I intend to
>> send it right away.
>
> Most likely you have a different setting in the 2 installed systems.
>
> I put these 2 lines (and some other defaults) in the AutoRun macro of the
> StartupMacros.txt file to make sure that I have the same in all the installs.
>  
> run("Colors...", "foreground=white background=black selection=yellow");
> run("Options...", "iterations=1 black pad edm=Overwrite count=1");
>
> This makes IJ to consider foreground as white (255) and background as black
> (0).
> Also if you start with a file that has an inverted LUT to start with, the
> results might not be what you expect.
>
> Make Binary and Convert to Mask can do different things depending on the
> settings of Make Binary.
>
> Cheers
>
> Gabriel
>

Thank you Gabriel.. that completed it and helped.

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