Re: Counting connected Droplets

Posted by Graeme Ball-2 on Aug 05, 2013; 11:13pm
URL: http://imagej.273.s1.nabble.com/Counting-connected-Droplets-tp5004315p5004317.html

maybe something like this macro?

run("8-bit");
setAutoThreshold("Triangle stack");
setThreshold(0, 251);
setOption("BlackBackground", true);
run("Convert to Mask");
run("Watershed");
run("Analyze Particles...", "size=300-750 circularity=0.3-1.00
show=Nothing display exclude clear include add");
roiManager("Show All with labels");
run("Summarize");

On Mon, Aug 5, 2013 at 11:27 PM, Michael <[hidden email]> wrote:

> Hello,
>
> I would like to count and measure droplets.
> Unfortunately all instructions i found so far didn't work since the drops
> are connected and therefore are not recognized as different objects, or the
> brighter middle of the drops causes problems.
> Example image:
> <http://imagej.1557.x6.nabble.com/file/n5004315/fc2_save_2013-07-10-140011-0000.png>
>
> Can someone please help me?
>
> Thank You!
>
> Michael
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/Counting-connected-Droplets-tp5004315.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
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