sequential batch subtraction

Posted by Mottlab on Aug 09, 2013; 4:55pm
URL: http://imagej.273.s1.nabble.com/sequential-batch-subtraction-tp5004370.html

Hi everyone, here's my situation:

I run tests on stomatal responses and aquire over 500 images per day in a single folder, and I need to create sequential difference images.

Basically, I need to batch subract image 1 from image 2, then image 2 from image 3, and image 3 from image 4, and so on for all 500 images, add false color, then save them all to a new folder.

Is there a plugin I could use, or a macro? I'm new to ImageJ and don't know where to begin.

Thanks for the help,
Lauren