Posted by
Adam Hughes on
Aug 12, 2013; 4:58pm
URL: http://imagej.273.s1.nabble.com/SIZE-MEASUREMENT-OF-PARTICLES-THESIS-DUE-TOMORROW-PLEASE-HELP-tp5004387p5004399.html
Im assuming since you drew the boundary, then you know the total area of
the boundary. If so, then by summing all of the area of the particles, you
will have the area of the spaces just by subtraction. If your goal is to
measure the area of this space and correlate it to the total size of the
bounding curve, then it doesnt make much sense to me to do it any other way.
On Aug 12, 2013 6:50 AM, "Jan Brocher - BioVoxxel" <
[hidden email]>
wrote:
>
> Hi Geology Guy, hi Christian,
>
> just a short comment on the last reply. The Feret's diameter is actually
the longest straight distance inside an image object (not the shortest
one!). ImageJ/Fiji also gives you the minimum feret, but this is also not
the smallest distance inside a feature, since the determination method kind
of ignores the (small) cavities in a particular object. Therefore, feret's
diameter is very usefull for certain analyses but you need to handle it
with care regarding specific data.
>
> Even though, strongly depending on your experiment and interpretation
(which might justify using e.g. feret's diameter), I would agree to
Christian and rather take the area if I want to compare it to the overall
area of the bigger feature (composed of the smaller ones). Because then,
you compare area with area.
>
> kind regards,
> Jan
>
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