Re: segmentation

Posted by Rob van 't Hof-2 on Aug 15, 2013; 2:51pm
URL: http://imagej.273.s1.nabble.com/segmentation-tp5004436p5004441.html

Hi,
Normally, go to image>adjust>color threshold. However, doing this
successfully depends rather a lot on staining /image quality. Do you
have an example?
Also is your section from demineralised bone, or undemineralised? In the
latter case there are a number of stains (such as von Kossa or Aniline
blue) that nicely stain only the mineralised tissue.
For more difficult segmentation have a look at the trainable WEKA
segmentation plugin. This is included in the Fiji distribution of ImageJ.

bye,
Rob


On 15/08/2013 15:04, Jędrzejas, Magdalena wrote:

> Hello,
>
> I would like to do a segmentation of a microscopy image using ImageJ. I want to divide the picture into three labels: bone (originally more or less yellow), cartilage (originally green) and connective tissue which has other colors. Then Id like to measure these three areas. What tool/plugin should I use? The contrast between the tissues is not so good.....
>
> I would really appreciate any help
>
> Magda
>
>
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