TrakEM: how to measure apposed surface area

Posted by Luisa de Vivo on Aug 21, 2013; 10:28pm
URL: http://imagej.273.s1.nabble.com/TrakEM-how-to-measure-apposed-surface-area-tp5004526.html

Hi,

I'd like to know whether in TrakEM there is the possibility to measure the
area of two apposed objects and save it as a separate object in the
project, in order to retrieve it in a second moment. I have not found a
clear explanation in the manual.

For instance, I have a stack of images where I'm segmenting a dendritic
spine and its terminal and I want to measure the area  of contact (and its
perimeter) between the two objects. I tried to trace a line between the pre
and post-synaptic objects using the brush tool and saving the segmentation
into an area list, but what I get at the end is not really an area but a
volume, even if I use the brush with minimum size. What do you suggest?

Thank you very much,

Luisa


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Luisa de Vivo, PhD
Department of Psychiatry
University of Wisconsin
6001 Research Park Boulevard
Madison WI 53719

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