Posted by
Johannes Koch on
URL: http://imagej.273.s1.nabble.com/HELP-needed-from-IMAGEJ-to-Photoshop-tp5004584p5004592.html
I am not sure, if I understand your question properly.
Please look up (just google it) the usability of LUTs (look-up tables).
Unless you record with an RGB camera, usually your images ARE grayscale.
For visibility reasons (i.e. looking simultaneously at 2/3 images aka
channels), the channels representing different fluorophores are
colorized. This is artificial and has absolutely no relevance.
When you save your ImageJ image with three DIFFERENT channels as tiff,
it is saved as multilayer tiff. I dont know what to do with that in
Photoshop, but a wild guess would be to work on the layer concept, put
each channel in a separate layer and apply the color (aka Lookup) you want.
Surely, it would be better to convert your image to RGB in ImageJ and
then save it. Again, my guess would be that the splitting operation in
photoshop would now represent the proper colors, given that you had R,G
and B as separate channels in the original image. If you had cyan,
magenta and yellow, then a RGB split would not work.
Maybe my answer provided some keywords that help you to look further.
cheers,
Johannes
*Dr. Johannes Koch*
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Am 28.08.2013 09:33, schrieb Duleep Samuel:
> if you can save as jpg in ImageJ then photoshop will open in color,Samuel,
> Bangalore, India
>
>
> On Wed, Aug 28, 2013 at 12:53 PM, Dionysios Lefkaditis <
[hidden email]
>> wrote:
>> Hi balloo02,
>>
>> While I am not a Photoshop user, I am pretty sure that what you are seeing
>> is correct. I will try to give you an answer in simple words. When you
>> split an image into its colour channels, they essentially become single
>> channel (greyscale) images. So when opening them in Photoshop, there is no
>> way that that application knows that the image opened used to be a colour
>> component of colour image. Are the images identical to each other, or they
>> are slightly different? Of course that heavily depends on what was your
>> original image. Try to merge the colour channel images back to a colour
>> image and check if that will give you your original image. Hope that
>> helps...
>>
>> cheers!
>>
>>
>> On Wed 28 Aug 2013 04:36:05 AM CEST, balloo02 . wrote:
>>
>>> Hi, I am in dire need of help with converting imagej files that are saved
>>> as TIFF files to viewing them in Photoshop. In IMAGEJ, my images have 3
>>> separate channels (blue, red, green). My problem is that when I view the
>>> split images in photoshop they are gre,. Instead of my split images
>>> being
>>> blue, red, or green like when I saved them as TIFF files from IMAGEJ they
>>> are now grey. Furthermore, when I open those same files "turned" grey back
>>> in image they are now grey instead of being their original color (blue red
>>> or green). Can someone please help? Thank you
>>>
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