http://imagej.273.s1.nabble.com/HELP-needed-from-IMAGEJ-to-Photoshop-tp5004584p5004597.html
It's easiest to convert your image to RGB in Image J. It would likely go
fastest. But if it is a fluorescence image, and you want to convert in
1. Convert to RGB Color (Image> Mode> RGB Color)
2. In the Levels dialog box, make the two channels you *don't* want to a
level of zero (0). For example, if you want the grayscale image to be Red,
Channels drop down and choose the Green channel. In the Output Levels
hand box. Then do the same with the Blue channel. This will leave you
and then hit the F key to do this to all other images. This can be done in
the Actions palette (Window > Actions). Or you can make what is called a
droplet (File > Automate > Create Droplet). You can create a droplet, put
> I am not sure, if I understand your question properly.
>
> Please look up (just google it) the usability of LUTs (look-up tables).
> Unless you record with an RGB camera, usually your images ARE grayscale.
> For visibility reasons (i.e. looking simultaneously at 2/3 images aka
> channels), the channels representing different fluorophores are colorized.
> This is artificial and has absolutely no relevance.
>
> When you save your ImageJ image with three DIFFERENT channels as tiff, it
> is saved as multilayer tiff. I dont know what to do with that in Photoshop,
> but a wild guess would be to work on the layer concept, put each channel in
> a separate layer and apply the color (aka Lookup) you want.
>
> Surely, it would be better to convert your image to RGB in ImageJ and then
> save it. Again, my guess would be that the splitting operation in photoshop
> would now represent the proper colors, given that you had R,G and B as
> separate channels in the original image. If you had cyan, magenta and
> yellow, then a RGB split would not work.
>
> Maybe my answer provided some keywords that help you to look further.
>
> cheers,
> Johannes
>
>
>
>
> *Dr. Johannes Koch*
>
> *Tissue Med Biosciences GmbH*
>
> Magnesitstrasse1 | A-3500 Krems
>
> T: +43/2732/87470-300 | M:
[hidden email] <mailto:
>
[hidden email]>
>
> *www.tmbiosciences.com <
http://www.tmbiosciences.com/**>*
>
> Diese E-Mail enthält vertrauliche und/oder rechtlich geschützte
> Information. Wenn Sie nicht der richtige Adressat sind oder diese E-Mail
> irrtümlich erhalten haben, informieren Sie bitte unverzüglich den Absender
> und vernichten Sie diese E-Mail sofort. Das unerlaubte Kopieren sowie die
> unbefugte Weitergabe dieser E-Mail sind nicht gestattet.
>
> This e-mail may contain confidential and/or privileged information. If you
> are not the intended recipient or have received this e-mail in error please
> notify the sender immediately and destroy this e-mail. Any unauthorized
> copying, disclosure or distribution of the material in this e-mail is
> strictly forbidden.
>
> Am 28.08.2013 09:33, schrieb Duleep Samuel:
>
> if you can save as jpg in ImageJ then photoshop will open in color,Samuel,
>> Bangalore, India
>>
>>
>> On Wed, Aug 28, 2013 at 12:53 PM, Dionysios Lefkaditis <
>>
[hidden email]
>>
>>> wrote:
>>> Hi balloo02,
>>>
>>> While I am not a Photoshop user, I am pretty sure that what you are
>>> seeing
>>> is correct. I will try to give you an answer in simple words. When you
>>> split an image into its colour channels, they essentially become single
>>> channel (greyscale) images. So when opening them in Photoshop, there is
>>> no
>>> way that that application knows that the image opened used to be a colour
>>> component of colour image. Are the images identical to each other, or
>>> they
>>> are slightly different? Of course that heavily depends on what was your
>>> original image. Try to merge the colour channel images back to a colour
>>> image and check if that will give you your original image. Hope that
>>> helps...
>>>
>>> cheers!
>>>
>>>
>>> On Wed 28 Aug 2013 04:36:05 AM CEST, balloo02 . wrote:
>>>
>>> Hi, I am in dire need of help with converting imagej files that are
>>>> saved
>>>> as TIFF files to viewing them in Photoshop. In IMAGEJ, my images have 3
>>>> separate channels (blue, red, green). My problem is that when I view the
>>>> split images in photoshop they are gre,. Instead of my split images
>>>> being
>>>> blue, red, or green like when I saved them as TIFF files from IMAGEJ
>>>> they
>>>> are now grey. Furthermore, when I open those same files "turned" grey
>>>> back
>>>> in image they are now grey instead of being their original color (blue
>>>> red
>>>> or green). Can someone please help? Thank you
>>>>
>>>> --
>>>> ImageJ mailing list:
http://imagej.nih.gov/ij/list.****html<
http://imagej.nih.gov/ij/list.**html>
>>>> <
http://imagej.nih.gov/**ij/list.html<
http://imagej.nih.gov/ij/list.html>
>>>> >
>>>>
>>>> --
>>> ImageJ mailing list:
http://imagej.nih.gov/ij/list.****html<
http://imagej.nih.gov/ij/list.**html>
>>> <
http://imagej.nih.gov/**ij/list.html<
http://imagej.nih.gov/ij/list.html>
>>> >
>>>
>>> --
>> ImageJ mailing list:
http://imagej.nih.gov/ij/list.**html<
http://imagej.nih.gov/ij/list.html>
>>
>>
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.**html<
http://imagej.nih.gov/ij/list.html>
>