Re: Using ImageJ to recognise synapses - very grateful for any help
Posted by
Gabriel Landini on
Sep 04, 2013; 4:32pm
URL: http://imagej.273.s1.nabble.com/Using-ImageJ-to-recognise-synapses-very-grateful-for-any-help-tp5004648p5004651.html
On Wednesday 04 Sep 2013 16:03:01 Alex Jeans wrote:
> I have got as far as thresholding the raw images and using the particle
> analyzer to define the puncta themselves. How to identify that subset which
> has overlap with another channel is the bit I am stuck on.
We have implemented a spatial reasoning logic model that could be used to find
this kind of relationships between binary objects across images (or channels
in a stack).
http://www.dentistry.bham.ac.uk/landinig/software/spatial/rcc8d.htmlThe IEEE PAMI paper listed in that page gives all the theoretical work.
Ask if you have any questions on this.
Cheers
Gabriel
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