Re: Using ImageJ to recognise synapses - very grateful for any help

Posted by Gabriel Landini on Sep 04, 2013; 4:45pm
URL: http://imagej.273.s1.nabble.com/Using-ImageJ-to-recognise-synapses-very-grateful-for-any-help-tp5004648p5004652.html

On Wednesday 04 Sep 2013 16:53:37 you wrote:
> If you have already thresholded your images, you can just simply combine the
> two thresholded images with an "AND" operator (Process>Image Calculator).
> You can then use the resulting image as the seed for a binary
> reconstruction using Gabriel Landini collection of morphological operators
> for ImageJ
> (http://www.dentistry.bham.ac.uk/landinig/software/software.html).
> Following the reconstruction that should restore the original size of the
> particles based on the image that you selected as the Mask image which
> contain an overlapping region.

That would miss regions that do not really overlap on the lattice, but
"should" overlap in the real world, like 45 degree (diagonal) lines that cross
at a 90 degree angle exactly at a corner (i.e. they do not share a pixels).
That is the PO* relation and shown in the link I posted earlier.  The AND of
those 2 lines returns an empty result. The RCC8D plugin can detect those
relations. Connectedness in square grids has some non-intuitive properties.

Cheers

Gabriel

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