Posted by
dscho on
URL: http://imagej.273.s1.nabble.com/HELP-needed-from-IMAGEJ-to-Photoshop-tp5004584p5004663.html
Dear balloon02,
On Tue, 27 Aug 2013, balloo02 . wrote:
> Hi, I am in dire need of help with converting imagej files that are
> saved as TIFF files to viewing them in Photoshop. In IMAGEJ, my images
> have 3 separate channels (blue, red, green). My problem is that when I
> view the split images in photoshop they are gre,. Instead of my split
> images being blue, red, or green like when I saved them as TIFF files
> from IMAGEJ they are now grey. Furthermore, when I open those same files
> "turned" grey back in image they are now grey instead of being their
> original color (blue red or green). Can someone please help? Thank you
Please note that the red/green/blue visualization of scientific images is
just a compromise, mostly for historical reasons. Scientific images are
usually multi-channel, and visualizing them as RGB is actually wrong:
imagine an image of green fluorescent cells, the first channel
representing a range of wavelengths -- smooshing them all into the same
green is obiously incorrect, I hope you agree. Worse: the vast majority of
computer screens is not calibrated, so it depends on the screen *which*
wrong green you see.
Further, the pixel values stored in scientific images are typically *not*
intensities as in photographs. See
http://www.4p8.com/eric.brasseur/gamma.html for the very real implications
of this issue.
And then there is also the issue of human vision: our color vision is
simply not very precise (as you can easily verify yourself at dusk: you
see gray with only minimal color).
Assuming that you want to present your multi-channel data to others, I
would therefore *strongly* suggest to keep the images in grayscale, but
color their labels. That way, you have a clear representation with minimal
information loss.
Ciao,
Johannes
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