Re: problem with Trackmate

Posted by David Michonneau on
URL: http://imagej.273.s1.nabble.com/problem-with-Trackmate-tp5004766p5004774.html

Le 12/09/13 17:59, Johannes Schindelin a écrit :

> Hi David,
>
> On Thu, 12 Sep 2013, David Michonneau wrote:
>
>> I'm trying to do cell tracking using the plugin "Trackmate" in Fiji. It
>> was used to work but since two days, I always have this error message
>> when launching Trackmate :
>> (Fiji Is Just) ImageJ 1.48b; Java 1.6.0_51 [64-bit]; Mac OS X 10.8.4; 1035MB
>> of 5991MB (17%)
>>
>> java.lang.NoClassDefFoundError: net/imglib2/meta/Metadata
> Sorry, my mistake. We fixed this last week in the source code, but I
> simply forgot to upload a new version to the update site. After an update
> with Help>Update Fiji, it should work now!
>
> Ciao,
> Johannes
Hi Johannes,

Thank you for your response. With the update, there is no more trouble
to launch Trackmate. However, there is still some problem to track
cells. I'm trying to track cells in a film with multiple z stacks. When
I use trackmate with these films, it doesn't detect any spot (i have
tried with different value of blobs diameter and threshold but it didn't
change anything). Then, I have tried to make a z project before
analysis. With a z project, Trackmate detects a lot of spots, but it
doesn't track any of them (the final result is a null track speed and
each spot is tracked as a motionless cells during the whole film). Do
you know how I could solve this problem ? Thank you,

David

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