Re: problem with Trackmate

Posted by fabrice senger-2 on Sep 13, 2013; 2:58pm
URL: http://imagej.273.s1.nabble.com/problem-with-Trackmate-tp5004766p5004776.html

Hi there,

I unfortunatly experience the same kind of problem. In my case I have a
time lapse in wich I track a single spot, it's my test stack and previously
this was always workinf fine.
By know I can launch trackmate but could find no way to find and track this
"easy" feature.
So there must be another problem.

For the time being is ther a simple way to go back o the previous version
of FIJI ?

Thank youvery much for your efforts,

Fabrice.


2013/9/13 David Michonneau <[hidden email]>

> Le 12/09/13 17:59, Johannes Schindelin a écrit :
>
>  Hi David,
>>
>> On Thu, 12 Sep 2013, David Michonneau wrote:
>>
>>  I'm trying to do cell tracking using the plugin "Trackmate" in Fiji. It
>>> was used to work but since two days, I always have this error message
>>> when launching Trackmate :
>>> (Fiji Is Just) ImageJ 1.48b; Java 1.6.0_51 [64-bit]; Mac OS X 10.8.4;
>>> 1035MB
>>> of 5991MB (17%)
>>>
>>> java.lang.**NoClassDefFoundError: net/imglib2/meta/Metadata
>>>
>> Sorry, my mistake. We fixed this last week in the source code, but I
>> simply forgot to upload a new version to the update site. After an update
>> with Help>Update Fiji, it should work now!
>>
>> Ciao,
>> Johannes
>>
> Hi Johannes,
>
> Thank you for your response. With the update, there is no more trouble to
> launch Trackmate. However, there is still some problem to track cells. I'm
> trying to track cells in a film with multiple z stacks. When I use
> trackmate with these films, it doesn't detect any spot (i have tried with
> different value of blobs diameter and threshold but it didn't change
> anything). Then, I have tried to make a z project before analysis. With a z
> project, Trackmate detects a lot of spots, but it doesn't track any of them
> (the final result is a null track speed and each spot is tracked as a
> motionless cells during the whole film). Do you know how I could solve this
> problem ? Thank you,
>
> David
>
>
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