Re: FWHM on line selection?

Posted by Unruh, Jay-2 on
URL: http://imagej.273.s1.nabble.com/FWHM-on-line-selection-tp5004777p5004780.html

The best option is a Gaussian fit (fwhm=2.35*stdev).  This is probably best done outside of ImageJ, but I also have a fit multi gaussian plugin for plots in my distribution:

http://research.stowers.org/imagejplugins/

Jay

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Vitha, Stanislav
Sent: Friday, September 13, 2013 10:22 AM
To: [hidden email]
Subject: FWHM on line selection?

I would like to  calculate FWHM from a line selection to measure microscope resolution.
My sample contains cytoskeletal filaments (similar to microtubules). I draw a line selection across the individual filament  and plot the intensity profile. From that profile I can eyeball the full width at half maximum (FWHM), but I would prefer an actual calculation.
I am aware of the FWHM plugin that can calculate FWHM of a spot in X and Y direction,  but since my filaments are running in random directions, I do not think I can use it.

Suggestions and hints would be appreciated.
Thanks!

Stan Vitha
Microscopy and Imaging Center
Texas A&M University



--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html