Re: FWHM on line selection?

Posted by LIM Soon Yew John (IMB) on
URL: http://imagej.273.s1.nabble.com/FWHM-on-line-selection-tp5004777p5004792.html

Hi Stanislav,

I had wrote an ImageJ macro that calculate the FWHM from a line ROI. This macro is attached in this e-mail. You will need to make a selection line and then run this macro.

Hope this helps.

Best Regards,
John

________________________________________
From: ImageJ Interest Group [[hidden email]] On Behalf Of Vitha, Stanislav [[hidden email]]
Sent: Friday, September 13, 2013 11:22 PM
To: [hidden email]
Subject: FWHM on line selection?

I would like to  calculate FWHM from a line selection to measure microscope resolution.
My sample contains cytoskeletal filaments (similar to microtubules). I draw a line selection across the individual filament  and plot the intensity profile. From that profile I can eyeball the full width at half maximum (FWHM), but I would prefer an actual calculation.
I am aware of the FWHM plugin that can calculate FWHM of a spot in X and Y direction,  but since my filaments are running in random directions, I do not think I can use it.

Suggestions and hints would be appreciated.
Thanks!

Stan Vitha
Microscopy and Imaging Center
Texas A&M University



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