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Re: Import image sequence convert to single channel

Posted by Holloway, Michael on Sep 16, 2013; 1:26pm
URL: http://imagej.273.s1.nabble.com/Import-image-sequence-convert-to-single-channel-tp5004790p5004805.html

Sorry again.  I quickly discovered batch converter.java and problem solved.
 But it does seem that the "convert to 8-bit" option in the import> image
sequence dialog is supposed to handle the RGB to single channel conversion.
 It doesn't.


On Mon, Sep 16, 2013 at 8:56 AM, Holloway, Michael <
[hidden email]> wrote:

> Thanks for the responses, but I errored in describing my problem.  I have
> a series of slices with only green label unfortunately saved as RGB.
>  Importing gives what you would expect, black images for red and blue
> interspersed with green.  Channel selection doesn't seem to work with an
> imported stack.  Just get back a message that I need to open a multichannel
> image.  What I need to know is if there's a batch method of converting my
> slice images prior to importing, or if there's a function to split the
> channels of the stack.  Can't find instruction on doing this in import
> directions, and can't find discussion of this elsewhere.
>
> Thanks,
> Mike Holloway
>
>
> On Mon, Sep 16, 2013 at 2:34 AM, Aryeh Weiss <[hidden email]> wrote:
>
>> On 9/16/13 6:32 AM, Holloway, Michael wrote:
>>
>>> Importing my tiffs gives a stack with empty red and blue images.
>>>  Selecting
>>> "convert to 8-bit" doesn't seem to do anything, and I can't locate a
>>> conversion utility.  I'm guessing I need something that will convert my
>>> RGB
>>> tiffs to a single channel.  Do I do this in batch before importing the
>>> sequence, or is there a way of fixing the stack?
>>>
>>>
>>>  If it is a stack of RGB images, you can use image>color>split channels
>> , then close the two empty channels.
>> If you have composite images, you can also split channels and then close
>> the empty channels.
>>
>> --aryeh
>>
>> --
>> Aryeh Weiss
>> Faculty of Engineering
>> Bar Ilan University
>> Ramat Gan 52900 Israel
>>
>> Ph:  972-3-5317638
>> FAX: 972-3-7384051
>>
>>
>

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