Excluding overexposured pixel from auto thresholding

Posted by pang on Sep 16, 2013; 7:32pm
URL: http://imagej.273.s1.nabble.com/Excluding-overexposured-pixel-from-auto-thresholding-tp5004810.html

Dear ImageJ colleagues,


Is there any way to exclude some pixels with over-exposed (for example, any pixel greater than 4000 for 12-bit camera) before applying OTSU autothresholding?  Any suggestion is highly appreciated.

I tried to avoid using create selection and then restore selection, but if it is the only method, I think I will have to use it.

Thanks,

Zhengyu
Zhengyu Pang, Ph.D.
Biochemistry and Bioanalytics Laboratory Life Sciences and Molecular Diagnostics Technology Organization Diagnostics and Biomedical Technologies GE Global Research, K1-5B37A One Research Circle Niskayuna, NY 12309
T: 518-387-4015
F: 518-387-7765

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