Re: Colocalisation

Posted by Paul Buchanan on Sep 19, 2013; 3:39pm
URL: http://imagej.273.s1.nabble.com/Colocalisation-tp5004854p5004858.html

Thanks Sonia and Michael,

I will try converting the images to 8bit and see how that goes.

I was really only trying to use JaCoP for object based co localisation. Is this the best plug in for that or is there any better methods out there? My output from JaCoP for object based colocalisation only seems to give me coordinates and nothing to with colocalisation. Any thoughts why?

Also with JaCoP the thresholding tab, i dont need to change any of the settings, as it should do it automatically is that correct?

Also Sonia yeah i have read that paper but this not being my main area parts of it i didnt completely understand, hence why am still unsure of the process involved in intensity based colocalisation and how it takes into account pixel location and also if these plugins take into account the whole 3d stack?

Thanks again for your help.

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