Re: Colocalisation
Posted by
Paul Buchanan on
Sep 20, 2013; 11:08am
URL: http://imagej.273.s1.nabble.com/Colocalisation-tp5004854p5004871.html
Is there a way to do the thresholding using another method and then use that with JaCoP?
I also noticed that costes sets the threshold to low.
Surely setting by hand isnt consistent if your analysising a lot of images?
What did you mean by "when all pixels in the nuciei are below threshold", what does that look like i.e. the pixels in the nuclei you cant see?
Thanks for your help again
Paul
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