Re: DeconvolutionLab Batch Processing

Posted by mehulmvora on Sep 20, 2013; 5:04pm
URL: http://imagej.273.s1.nabble.com/DeconvolutionLab-Batch-Processing-tp5004865p5004882.html

Hi Brian

Thank you for your reply. I'm a complete newbie to deconvolution and confocal microscopy.

I don't have individual PSF files for each of the images - isn't that what's required? Each image is different from the rest (but they were taken at the same time with the same scope and settings). Will one PSF file work for them all? I thought that each image will have it's own psf?

Thank you!

On Fri, Sep 20, 2013 at 12:52 PM, bnorthan [via ImageJ] <[hidden email]> wrote:

Hi

Do you have the PSF image open?? I've done this before and I needed to open
the PSF image, go to the PSF option, then select "Flip PSF Quadrants" (if
the PSF is centered).  After that select the input and output folders.

It should then process all the files using the same PSF.

Brian


On Thu, Sep 19, 2013 at 5:52 PM, mehulmvora <[hidden email]> wrote:

> Hello
>
> I wish to use DeConvolutionLab to perform batch processing on a number of
> .tiff stacks.
>
> I can choose the Input and Output folders without a problem. When I hit RUN
> it will show me the Initial input files and the new output file names.
>
> However, it asks me for PSF file.
>
> When I do them individually, I use the same file as both input and output.
> But am not sure how to do this for batch?
>
> Any help appreciated. I have 100s of files to process ! :)
>
>
>
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