Posted by
Benito Alarcon on
Sep 25, 2013; 9:32am
URL: http://imagej.273.s1.nabble.com/area-measurment-tp5004928p5004932.html
>
>
>Date: Wed, 25 Sep 2013 02:14:15 +0200
>From: Thuraya Medhat <
[hidden email]>
>Subject: area measurment
>
>please i need help how to measure area
>[image: Inline image 1]
>i followed these steps:
>1:split channels
>2: worked on the green one, then i made subtract for the background
>3: from image i choose adjust then threshold
>4: then from
>5: edit, selection , create selection
>6: analyse, set measurement ( checked limit to threshold and area and
>directed to the green photo)
>
>7: analyse, measure where the problem is,,, the read i got is the whole
>image area
>and when i use anaylse particles instead it doesn't give me a correct
>result at all as sometimes i got the area as 100 %
>
>please help me
Hello Thuraya, I hope this help you:
first I would set the scale of the image (Analyze; Set
Scale). Then do your steps and measure your selection. The value of
%Area represent the percent of the area occupated by pixels with 255.
If you multiply the value of the Area (total selection area) by %Area
(which is the percent of that area with a value of 255) divide by 100
you get the area of the signal.
Benito Alarcon
Instituto de Biomedicina de Valencia
CSIC. Spain
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