Re: Selecting a masked/overlayed object

Posted by ntse163 on Sep 26, 2013; 12:48am
URL: http://imagej.273.s1.nabble.com/Selecting-a-masked-overlayed-object-tp5004930p5004944.html

Hi,
I cannot draw the region of interest I require as this removes the 'mask' or outline of the area that I created, in the 2nd channel. If i do this in reverse and draw the region of interest first, the region of interest is removed when I create the mask. I need to keep the mask and draw the region of interest. Would this be possible?

If not, is there an alternative to masking the area that I could use? I need to select the exact area of staining in a region of interest in one channel and apply this area to the other channel. I am trying to determine the amount of staining, in the region of interest, in the 2nd channel that corresponds to the staining in the 1st channel