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Segmentation of muscle fiber

Posted by Pascal Lorentz-2 on Sep 30, 2013; 11:18am
URL: http://imagej.273.s1.nabble.com/Segmentation-of-muscle-fiber-tp5004976.html

Dear List

I got this Image of muscle fiber (Fiber-I white and Fiber-II red) from a
user:

http://www.picfront.org/d/91MZ

She wants to compare white versus red cells. She needs the percentage of
cells (white vs red) but only cells that have a circularity between 0.6
and 0.8 should be considered. Truncated cells should be excludes as
well.  I am able to seperate white and red cells but just area wise. I
would be glad if anybody as a good procedure to segment the single cells
(if possible at all).

Thanks a lot for your help

Pascal

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Pascal Lorentz
BioOptics Facility
Department of Biomedicine
University of Basel
Mattenstrasse 28
4058 Basel
Switzerland

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