Re: Segmentation Problem

Posted by Cammer, Michael on Oct 03, 2013; 4:08pm
URL: http://imagej.273.s1.nabble.com/Segmentation-Problem-tp5005009p5005027.html

I tried converting to HSV, media filtering, and segmenting each with simple threshold or find edges / variance  and use logical AND to narrow to the white cells. Isolating the white cells from everything else is simple but I couldn't get the cells to separate from each other.  If anybody else has a method, I'd be very interested to hear it too.

But if she has fewer than 1000 or so cells, I'd just trace them.

Regards,

Michael



-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Pascal Lorentz
Sent: Wednesday, October 02, 2013 7:54 AM
To: [hidden email]
Subject: Segmentation Problem

Dear List

I got this Image of muscle fiber (Fiber-I white and Fiber-II red) from a
user:

http://www.picfront.org/d/91MZ

She wants to compare white versus red cells. She needs the percentage of cells (white vs red) but only cells that have a circularity between 0.6 and 0.8 should be considered. Truncated cells should be excludes as well.  I am able to seperate white and red cells but just area wise. I would be glad if anybody as a good procedure to segment the single cells (if possible at all).

Thanks a lot for your help

Pascal

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