Login  Register

Re: Pls help me with segmentation issue to count cell colonies in 3D agars.

Posted by Peter Haub on Oct 06, 2013; 7:04pm
URL: http://imagej.273.s1.nabble.com/Pls-help-me-with-segmentation-issue-to-count-cell-colonies-in-3D-agars-tp5004983p5005057.html

Hi Johannes,

Thanks for your comments and correction about artifacts in JPEG images.
You are right. 'Clearly' was not that clear.
I wish there would be a link to some background information on this
topic. And more a kind of "Do's and Dont's in Scientific Imaging" where
beginners could be guided to and where they can find the most relevant
and important info in short.

Do you have anymore idea beside the very nice books of Burger&Burge (
http://imagingbook.com ) and also Peter Bankhead (
http://blogs.qub.ac.uk/ccbg/files/2013/06/Analyzing_fluorescence_microscopy_images.pdf 
), where the aspects of jpeg compression is described?

Regards,
Peter

On 04.10.2013 14:50, Johannes Schindelin wrote:

> Hi Peter,
>
> On Fri, 4 Oct 2013, Peter Haub wrote:
>
>> Just one more hint: Saving scientific images in jpg-format is not
>> optimal. You will lose information and introduce jpg-artefact which are
>> clearly visible in your images and which are sub-optimal for every
>> analysis process.
> Often, those artifacts are not so clearly visible (JPEG format was
> designed to fool our eyes, after all...) unless after processing -- and
> then often only to the trained eye.
>
> But artifacts they are, therefore JPEG is a poor format to store
> scientific images in. It is appropriate only in a very narrow set of
> bandwidth-constrained visualization tasks, and then only for transmitting,
> never for storing nor analyzing.
>
> Ciao,
> Johannes
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html