correction for stray light on epifluorescence images

Posted by Stoyan Pavlov on Oct 16, 2013; 7:10am
URL: http://imagej.273.s1.nabble.com/correction-for-stray-light-on-epifluorescence-images-tp5005196.html

Dear all,
I've got epifluorescence images, on which I am to quantify the total
fluorescence (integrated density) of ROIs. On some of the images there are
bright glowing artifacts. I exclude them from measurement however the stray
light from them affects the neighbouring pixels. So i am searching for a
way to neutralize this influence.
My images are corrected for illumination irregularities (shading
correction) at the acquisition.
I was wondering would it be legitimate to do this correction dividing all
my images by their smoothed with a big median filter (>100px) versions. I
would do this using the Image calculator plus divide formula (i1/12)*k1
where k1 would be the maximum grey value of the smoothed version.

Best regards!

Stoyan Pavlov
---
Dr. Stoyan P. Pavlov, MD, PhD
Departament of Anatomy, Histology and Embryology
Medical University "Prof. Dr. Paraskev Stoyanov", Varna
Prof. Marin Drinov Str.55
9002 Varna
 Bulgaria
Tel: +359 (0) 52 - 677 - 052
e-mail: [hidden email]

Zentrum für Anatomie
Uniklinik Köln
[hidden email]
Tel: [phone]

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