deleting uneventful frames in a tif stack

Posted by jehangir cama on Oct 18, 2013; 10:00am
URL: http://imagej.273.s1.nabble.com/deleting-uneventful-frames-in-a-tif-stack-tp5005238.html

Hi,

I would appreciate any help with this- I have data stored as tif stacks
(eg., a single tif file with 5000 images saved). I want to define two
regions of interest that I can define in the first frame, and which stay
the same throughout the 5000 frames.

Each roi is bright except when dark (approximately) circular objects pass
through (these are the events I want to study). I want to use ImageJ to
delete all the frames in which these dark objects are* absent* (so I want
to modify the tif stack to only keep those frames in which I have these
events).

Is this possible in ImageJ? Ideally, I need to also detect the radius and
intensity of the objects, but the first thing I need to do is get rid of
the extra frames. I'm new to using macros/plugins so if there is anything
close to what I need, I would be grateful for a link.

Thanks and regards

Jehangir

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html