Posted by
Jacqueline Ross on
Oct 31, 2013; 10:29pm
URL: http://imagej.273.s1.nabble.com/Macros-for-Colour-Deconvolution-plugin-saving-multiple-files-tp5005383p5005411.html
Hi Gabriel,
It's a modified Picro Sirius red/ light Fast green combination. Shows collagen in red and everything else is green. The collagen can also be viewed with polarised light.
One of our researchers developed it based on one by John Kiernans in Canada. Works well.
Cheers,
Jacqui
Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND
Tel: 64 9 923 7438
Fax: 64 9 373 7484
http://www.fmhs.auckland.ac.nz/sms/biru/-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Gabriel Landini
Sent: Thursday, 31 October 2013 10:08 p.m.
To:
[hidden email]
Subject: Re: Macros for Colour Deconvolution plugin - saving multiple files
On Thursday 31 Oct 2013 03:20:49 you wrote:
> just replace the line for the vectors that we use ("vectors=[User
> values] show [r1]=60.27969 [g1]=15.12007 [b1]=14.169498 [r2]=53.738953
> [g2]=114.16744 [b2]=106.36251 [r3]=1.4947393 [g3]=7.050659
> [b3]=12.738025");) with your own choice.
Just curious, what stain is that?
Regards
Gabriel
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