Posted by
carlo bianco on
Nov 08, 2013; 12:51pm
URL: http://imagej.273.s1.nabble.com/Trouble-quantifying-neurite-outgrowth-from-explant-tp5005499p5005502.html
In my opinion you can try
an efficient thresholding (invert+manual tresholding)
followed by manual selection of roi. there is also the ad hoc plugin
http://www.imagescience.org/meijering/software/neuronj/but I never used it, and seems to be more useful.
With an other approach you can use grids and assess the presence/absence
of dendritic branch (is a systematic sampling )
If you extract the boundary and skeletonize it, you can evaluate the compelxity of branching
by means on fractal box counting, the fractal dimension
can be viewed as a space filling capacity syntetic indicator
and is used in quantitative neuropathology.
best wishes for your research
carlo bianco
Bologna University
Il Venerdì 8 Novembre 2013 12:39, Marcus_pocus <
[hidden email]> ha scritto:
Dear all,
I'm very new to the imageJ software and am having quite a bit of trouble
finding a time efficient way to measure three parameters in the following
type of image.
<
http://imagej.1557.x6.nabble.com/file/n5005499/Expl_2_ipsi_-_photo_for_imageJ_forum.jpg>
The parameters I'm looking to measure are:
- Surface covered by the neurites
- Neurite density
- Average neurite lenght
Any help of advice would be greatly appreciated!
Cheers,
MP
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