http://imagej.273.s1.nabble.com/Trouble-quantifying-neurite-outgrowth-from-explant-tp5005499p5005505.html
> Thanks for your answer!
>
> The problem is that the images aren't clear enough to totally exclude the
> background and specks and include all the neurites, so use of NeuronJ or the
> skeletonize function aren't possible (unless I'm mistaken). I'm currently
> doing immunofluorescence stainings in order the obtain better images.
>
> As for these brightfield pictures, I was initially thinking of something
> along the line of this:
> <
http://imagej.1557.x6.nabble.com/file/n5005503/Expl_2_ipsi_-_photo_for_imageJ_forum_2.jpg>
> Selecting 2 ROIS corresponding to the explant and the halo of neurites and
> measuring the *overall area *that is covered by the neurites, the *optical
> density* of the "neurite zone" and the *average distance* between the two
> ROIs. All these being quite indirect measures of neurite outgrowth but still
> better than nothing.
>
> I imagine that measuring the *area *and the *OD *must be quite
> straightforward however I don't have a clue as to how I could measure the
> "average neurite lenght". Any tips?
>
> Thanks once more to any benevolent helpers!
>
> Cheers,
>
> Marc
>
>
>
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