Re: macro to plot multi color profiles

Posted by Krs5 on Nov 09, 2013; 2:15pm
URL: http://imagej.273.s1.nabble.com/macro-to-plot-multi-color-profiles-tp5002959p5005518.html

Hi Putri,

This seems to be a specific problem with lsm files. The macro works fine when using for example the confocal series from the ImageJ samples. If you use a LUT table for each individual channel (Image > Lookup Tables and select for each channel the colour you want) on your lsm files the problem is solved. I am not sure but it almost looks like the lsm channels somehow don't report the colour in the channel with as results a black line for each channel in the graph.

Best wishes

Kees

________________________________________
From: ImageJ Interest Group [[hidden email]] On Behalf Of chin [[hidden email]]
Sent: 09 November 2013 02:21
To: [hidden email]
Subject: Re: macro to plot multi color profiles

Hi Kees,

I used the macro on a 4 channels .lsm image. But  obtained 4 plots with only
"black" color. Even I
changed image to a composite form, the macro could show 4 channels on "which
channels do you want
to plot?". And it also obtained 4 plots with black color. Could you please
tell me what's wrong with my image or the operating steps i missed? Thanks
in advance.

putri <http://imagej.1557.x6.nabble.com/file/n5005516/1.jpg>



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