Re: Uneven illumination of background
Posted by
Gabriel Landini on
URL: http://imagej.273.s1.nabble.com/Uneven-illumination-of-background-tp5005529p5005531.html
On Monday 11 Nov 2013 03:01:25 thr rui wrote:
> I appreciate any suggestion.
That approach will, unfortunately, not work.
Images need to be corrected using the original background illumination as you
do not know what are the characteristics of the illumination source. Not only
intensity but you get a change of colour which is most likely not homogeneous
across the field.
See this:
http://imagejdocu.tudor.lu/doku.php?id=howto:working:how_to_correct_background_illumination_in_brightfield_microscopyIf you do post-hoc correction, you will end up with something that might look
better, but colour deconvolution is sensitive to this and will give you wrong
results.
Cheers
Gabriel
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