Re: Uneven illumination of background

Posted by Gabriel Landini on Nov 12, 2013; 10:10pm
URL: http://imagej.273.s1.nabble.com/Uneven-illumination-of-background-tp5005529p5005547.html

On Tuesday 12 Nov 2013 19:21:19 you wrote:
> Is it wrong to process the color deconvolution for uncorrected background
> images to just "help" segment specific stain color regions?

No it is not right. As explained here:
http://www.dentistry.bham.ac.uk/landinig/software/cdeconv/cdeconv.html
you need to correct the images.

I hope you read the link I sent last time. That explains everything you need
to do a transmittance background correction.

If you are following that method and still the images have the wrong colour,
one reason could be using a camera in auto exposure mode? That was also
explained in the link I posted.

Cheers

Gabriel

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