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Measuring images in screen pixels

Posted by Elkee on Nov 22, 2013; 8:14pm
URL: http://imagej.273.s1.nabble.com/Measuring-images-in-screen-pixels-tp5005667.html

I am measuring bacteria ratios for various species and I need to be able to make several measurement at the center and quarter marks of the diameter.  I did a macro to get the quarter points along the diameter to make the three other horizontal measurements when I hit draw.  This works perfectly on my larger bacteria with very clear images, but this width is too large when I get to my smallest bacteria.  I have found that the line segment tool is the perfect size for all my measurements and line widths, but I believe it is in screen pixels rather then image pixels.  I have drawn a picture that shows what I need, but I need it done at the thickness of the line segment tool (on the right) for smaller bacteria.