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Performing manual segmentation

Posted by Kevin Keraudren on Nov 30, 2013; 1:22pm
URL: http://imagej.273.s1.nabble.com/Performing-manual-segmentation-tp5005745.html

Hi,

I would like to manually segment a few cell images in order to evaluate
an automated segmentation (for instance trace the cell boundaries and
the contour of the nuclei).

I usually do manual segmentations with ITK-snap on medical images, not
microscopy images, but I think it is overkill for PNG/TIFF images, and
not appropriate to handle classes: I would like to have the cytoplasm
and the nuclei classes, and assign labels of the form cytoplasm-1,
cytoplasm-2, cytoplasm-3,... and nuclei-1, nuclei-2, nuclei-3.
For my application, I would have more classes than just cytoplasm and
nuclei.

Could I use ImageJ for this? Otherwise would you know of an appropriate
program for this kind of manual segmentation. In my mind it would be a
tool that allow you to segment the image with layers, as the layers of
Photoshop or GIMP, and each layer would correspond to a class.

Thanks a lot for your help,

Kind regards,

Kevin

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