Posted by
Albert Cardona-2 on
Dec 02, 2013; 6:33pm
URL: http://imagej.273.s1.nabble.com/TrakEM2-registration-of-new-images-tp5005767p5005774.html
2013/12/2 Ashwin <
[hidden email]>
> Hi All,
>
> I have a trakEM2 project that has is registered (stitched tiles in xy and
> aligned in Z) to which I want to add new images. Would I have to start from
> scratch and re-import all images to a new project and proceed with
> registration or could I integrate it with the existing project. To make
> things hard, I have some treeline object that have been traced in the older
> registered version, would I have to re-trace these treeline objects as
> well?
>
> I am not sure how to approach this issue, any help would be great.
>
> Thanks,
> Ashwin
>
Hi Ashwin,
here's what I would do:
A) If you will add images in new layers rather than in existing layers:
1. Lock all existing images.
2. Add the new images, which will be in new layers at the end.
3. Register from the last (locked) layer to the end.
While not "perfect", this will give you a reasonable volume to work with.
B) If you will add images into exiting layers, or want a "perfect"
registration:
1. "Save As" to a different XML file
2. Open the new one and remove the treelines and other annotations
3. Add the new images
4. Re-register all.
5. Open the old XML
6. Right-click on the Object Tree of the old and choose "Send to - Sibling
project...", which will transfer the treelines from the old to the new,
following the appropriate transformations of the underlying images.
The "sibling project" approach works because the old layers and images have
the same IDs in the new volume, having the latter been created with "Save
As".
Albert
--
http://albert.rierol.nethttp://www.ini.uzh.ch/~acardona/--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html