Posted by
Krs5 on
Dec 08, 2013; 12:52pm
URL: http://imagej.273.s1.nabble.com/Selecting-and-measuring-bvs-tp5005837p5005841.html
Dear Simon
If you split your channels (Image> Color > Split Channels) and subtract the blue channel from the red (Process > Image Calculator and select the proper images) you get an image with only your red areas. So these can easily be selected via threshold and added to the ROI manager (via Analyze>Analyze particles (set limits if necessary and flag Add to Manager). This will give your red selection.
Yes, you can Image properties for this or Analyze > Set Scale.
Best wishes
Kees
Dr Ir K.R. Straatman
Senior Experimental Officer
Centre for Core Biotechnology Services
University of Leicester
http://www.le.ac.uk/biochem/microscopy/home.html-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Simon Locke
Sent: 08 December 2013 04:24
To:
[hidden email]
Subject: Selecting and measuring bvs
Hi
I am trying to measure the Bvs shown above. They represent "neovascularity"
in symptomatic tendons. I look for the maximum that occurs with the pulse.
Is there a way to automate "red" area selection? I can leave it in a RGB format.
Is there an alternative to free hand selections and using ROI manager?
Unfortunately, I am not familiar with writing JAVA script and am limited to tools.
When I change the area from pixels to cms, do I just change cm in image properties?
Thank you in anticipation. I apologise for being limited in developing but I am foremost a clinician.
Dr Simon Locke
MBBS BMed Sci FACSP FFSEM (UK) FRACGP
Sport and Exercise Physician
Bendigo Orthopaedic and Sports Medicine Clinic
176 Barnard st, Bendigo, Victoria, 3550
P:613 5442 5288 F:613 5442 5399 W:sportmovementcentre.com
Centre for Sport+ Movement is now on Facebook
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