Re: Conifer needle surface area minus shadows

Posted by Shameem Sampath on Dec 09, 2013; 3:02am
URL: http://imagej.273.s1.nabble.com/Conifer-needle-surface-area-minus-shadows-tp5005845p5005848.html

Hi Tree-Doc,
I have a suggestion. Please accept my apologies if you have already explored this avenue :
 (a) Take a pic using a polarising filter to reduce the reflected glare.
(b). Follow the pathway you have described.

Kind regards,
Shameem Sampath MD
Consultant Knee Surgeon
The Bluespot Knee Clinic
Manchester
England
www.MrSampath.com



Sent from my iPhone

> On 8 Dec 2013, at 23:03, Tree-Doc <[hidden email]> wrote:
>
> I have about 250 scans of conifer tree needles and need to measure their
> surface area. I've been using Threshold Colour to adjust the HSB to remove
> part of the shadow, but I notice that depending on the exact threshold
> numbers I use, my resulting area calculation will vary by 25% or more (not
> good).
>
> Is there a better way to do this to achieve more accurate results?
>
> My methods: Adjust HSB to remove as much of the shadow as I can without
> removing green needle.
> Converting to binary.
> Despeckling the noise.
> Process>Binary>Close to capture the white center streaks in the needles.
> Filling the Holes.
> And analyzing particles
>
> Here is an example of the images I'm working with. I know the scans aren't
> ideal, but capturing better images in not an option.
> <http://imagej.1557.x6.nabble.com/file/n5005845/4L0001_exp.jpg>
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/Conifer-needle-surface-area-minus-shadows-tp5005845.html
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>
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