Posted by
Adam Hughes on
Dec 12, 2013; 8:25pm
URL: http://imagej.273.s1.nabble.com/Shadows-leaf-morphology-problem-tp5005891p5005892.html
You can easily set the size threshold when you do AnalyzeParticles in
ImageJ.
The real quesiton is how do you best pre-process the image such that it
makes the particles better discernable, which is pretty much the central
problem in digital image processing in general.
Can you send an example image directly so that we can have a look?
On Thu, Dec 12, 2013 at 2:29 PM, Leaf Pollution <
[hidden email]
> wrote:
> Hi there,
>
> I hope someone can help. I am carrying out my undergraduate dissertation on
> pollution on certain leaves. I have used an environmental scanning electron
> microscope to take photos of the particles at various magnifications. I
> want
> to count particles of certain size fractions (all below 10um) and hence use
> the threshold function to count particles. However, the morphology of the
> leaf is problematic. Ridges and dips are appearing as white/black and are
> hence being counted in particle analyzer. I am using a method similar to
> Ottele et al. (2012)
>
http://www.sciencedirect.com/science/article/pii/S0925857409001098 but my
> images are not as simple as these produced in the paper. Would anyone know
> what I need to try?
>
> Thanks so much In advance!
>
>
>
>
> --
> View this message in context:
>
http://imagej.1557.x6.nabble.com/Shadows-leaf-morphology-problem-tp5005891.html> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html