Re: [Software Feedback] source code for "intensity macro for background subtraction"

Posted by Michael Schmid on Dec 22, 2013; 12:34pm
URL: http://imagej.273.s1.nabble.com/Re-Software-Feedback-source-code-for-intensity-macro-for-background-subtraction-tp5005961p5005967.html

Hi Bryne,

If you have a roi with the background and the background varies rather
slowly, you can try to fit a polynomial:

http://imagejdocu.tudor.lu/doku.php?id=plugin:filter:fit_polynomial:start

Michael

______________________________________________________________________

 On Fri, Dec 20, 2013 at 7:21 PM, Bryne Ulmschneider
 <[hidden email]>wrote:

> Thanks, but that wasn't actually exactly what I was looking for. The
> rolling ball radius algorithm is the wrong thing to use for the type of
> image analysis that I'm using.
> this:
> http://loci.wisc.edu/software/intensity-macro-background-subtraction
> sounds more like what I want to do, but it doesn't work with
> non-brightfield images apparently. I tried the method you outlined above
> to
> get the source code and got this error message "Unable to display source
> for this plugin".
>
> I also tried looking on github using this link:
> https://github.com/uw-loci/misc-plugins and still wasn't able to find
> exactly what I'm looking for.
>
> Alternatively- basically what I'm trying to do is just do a background
> subtraction using an ROI with a 3-color hyperstack so if you know of an
> efficient way to do that, that would be useful.
> Thanks!
> -Bryne
>
> ------------------------------------------------------------
> Bryne Ulmschneider, PhD Candidate
> University of California, San Francisco
> Department of Anatomy, Nystul Laboratory
>

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