Posted by
hesham977 on
URL: http://imagej.273.s1.nabble.com/DAB-quantification-tp5006159p5006160.html
very interesting questions
please we in-need the reply in steps
we need to follow steps from the start of counting the positive cells in
the immunohistochemistry slides until the full results
Thanks in advance
On Fri, Jan 17, 2014 at 6:42 PM, Panayiota Ploutarchou <
[hidden email]> wrote:
> Hi everyone,
>
> Would love your feedback on an issue I'm having.
>
> So, I've done Immunohistochemistry on tissue sections, and finished with
> DAB staining (which basically gives brown staining at the locations where a
> reaction with the antibody took place). Now, I would like to quantify that,
> but the pixel intensity function of Image J seems to give weird results. So
> basically firstly I would like to know what this function actually does
> (i.e. does it measure dark pixels only? what about taking into account the
> shades of colour?) and also how would you suggest I go about quantifying
> the DAB staining as I would need to compare staining in control and treated
> sections.
>
> Many thanks for your help,
> Yiota
>
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