Re: BrdU cell counting
Posted by
Gabriel Landini on
Jan 17, 2014; 4:04pm
URL: http://imagej.273.s1.nabble.com/BrdU-cell-counting-tp5006086p5006161.html
On Friday 17 Jan 2014 07:48:58 you wrote:
> The size of the cells is not supposed to change between conditions,
> therefore the number of the cells I need is just to estimate the final
> number of cells between conditions.
I do not mean the "size of cells", but the "size of the differences" you are
expecting to find in whatever you are measuring (number, sizes or any other
quantity).
Choosing arbitrary sample sizes is a bad idea when you have the opportunity of
doing an a priori power analysis.
Why? because at the end of experiments somebody *will* ask you to do a
posteriori power analysis and you risk facing that to find the differences you
are looking for needs way more data than you collected.
Like many things, ignoring the problem does not make it go away.
Cheers
Gabriel
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