> Hi everyone,
>
> Would love your feedback on an issue I'm having.
>
> So, I've done Immunohistochemistry on tissue sections, and finished with
> DAB staining (which basically gives brown staining at the locations where a
> reaction with the antibody took place). Now, I would like to quantify that,
> but the pixel intensity function of Image J seems to give weird results. So
> basically firstly I would like to know what this function actually does
> (i.e. does it measure dark pixels only? what about taking into account the
> shades of colour?) and also how would you suggest I go about quantifying
> the DAB staining as I would need to compare staining in control and treated
> sections.
>
> Many thanks for your help,
> Yiota
>
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