Re: DAB quantification

Posted by Panayiota Ploutarchou on
URL: http://imagej.273.s1.nabble.com/DAB-quantification-tp5006159p5006163.html

Hi Esteban,

Just DAB!

Yiota
________________________________
From: G. Esteban Fernandez [[hidden email]]
Sent: Friday, January 17, 2014 4:19 PM
To: [hidden email]; [hidden email]; Panayiota Ploutarchou
Subject: Re: DAB quantification


Are your slides also stained with a counterstain like hematoxylin or only with DAB?

-Esteban

On Jan 17, 2014 7:54 AM, "Panayiota Ploutarchou" <[hidden email]<mailto:[hidden email]>> wrote:
Hi everyone,

Would love your feedback on an issue I'm having.

So, I've done Immunohistochemistry on tissue sections, and finished with DAB staining (which basically gives brown staining at the locations where a reaction with the antibody took place). Now, I would like to quantify that, but the pixel intensity function of Image J seems to give weird results. So basically firstly I would like to know what this function actually does (i.e. does it measure dark pixels only? what about taking into account the shades of colour?) and also how would you suggest I go about quantifying the DAB staining as I would need to compare staining in control and treated sections.

Many thanks for your help,
Yiota

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